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KMID : 0381120230450111367
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Genes and Genomics
2023 Volume.45 No. 11 p.1367 ~ p.1376
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ST6GALNAC1 promotes the invasion and migration of breast cancer cells via the EMT pathway
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Yunzhao Luo
Heng Cao
Chuqi Lei
Jun Liu
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Abstract
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Methods : Plasmids with siRNA were used to construct ST6GALNAC1 knockoff (si-ST6GALNAC1) MDA-MB-231 and MDA-MB-453 cells, while lentiviruses were used to construct ST6GALNAC1 over-expression (oe-ST6GALNAC1) MCF-7 and BT474 cells. Transfer efficiency was verified by Western Blot. Then we selected transfected cells and assessed the changes in cell proliferation, invasion, migration, and EMT markers.
Results : The expression of ST6GALNAC1 significantly enhanced cell migration and invasion, which was confirmed by Wound Scratch Assay and Transwell Assay. Particularly, ST6GALNAC1 expression directly induced the EMT signaling pathway. E-cadherin was markedly decreased in oe-ST6GALNAC1 cells, accompanied by an up-regulation of mesenchymal markers including N-cadherin, snail, and ZEB1. However, no significant correlation was found between ST6GALNAC1 expression and cell proliferation. All of the outcomes were reversely validated in si-ST6GALNAC1 cells.
Conclusions : The expression of ST6GALNAC1 promotes cell migration and invasion probably by triggering the molecular process of the EMT pathway in breast cancer cells, which may provide new clues for designing novel molecular targeted drugs in breast cancer treatment.
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KEYWORD
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O?glycosylation, ST6GALNAC1, Breast cancer cell lines, Epithelial-mesenchymal transition, Signal pathway
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